Get Clear-cut Photometric Results From Less Than Clear-cut Samples
Vet Lab Supplies > Get Clear-cut Photometric Results From Less Than Clear-cut Samples

Get Clear-cut Photometric Results From Less Than Clear-cut Samples

| by Les Ellison
Get Clear-cut Photometric Results From Less Than Clear-cut Samples

LipoClear Tubes

Veterinary laboratories are expected to get perfect diagnostic results from less than perfect blood samples. LipoClear tubes out-perform time consuming solvent based clean-ups to give photometrically clear, lipoprotein free samples in a matter of minutes.

Diagnostic tests based on photometric analysis need optically clear samples. These tests check the health of active proteins (enzymes) by measuring the change (absorbance) in a beam of light passing through the sample. Any light-absorbing contaminant will give a false reading that might lead to an incorrect diagnosis and ineffective treatment.

Hyperlipaemia can also be a serious problem

High levels of low-density lipoproteins (chylomicrons) in the blood cause opaque or cloudy serum samples. Often due to an animal's health or diet, this hyperlipaemia can also be a serious problem in poorly stored blood samples arriving at the laboratory long past their best. To make matters worse, these samples are often haemolysed – evidenced by the dark red-brown colour of haemoglobin breakdown.

Whatever the cause, the turbidity created by fatty-proteins and haemoglobin must be removed before photometry can be trusted. In the past, blood and serum samples were cleared using a time-consuming and potentially lethal mixture of solvents such as chloroform and phenol, together with powerful and unpleasant synthetic detergents. These chemicals were not only dangerous for laboratory staff, but risked a significant waste disposal hazard.

Pre-measured clearing reagent to treat 0.5ml of haemolysed or hyperlipaemic serum or plasma

LipoClear Tubes require no organic solvents, as the clearing process is entirely water based.  Each of the 1.5ml plastic centrifuge tubes in the 10-test or 40-test kit contains enough pre-measured clearing reagent to treat 0.5ml of haemolysed or hyperlipaemic serum or plasma. 5 minutes at room temperature starts the process and a short spin in a standard veterinary centrifuge, equipped to take 1.5ml centrifuge tubes, completes the clean-up.

At the end of the spin, the heavier matter will have formed a solid pellet at the bottom of the tube while the less dense material forms a floating plug at the surface. Carefully tilting the tube to pour off the cleared liquid will leave the pellet fixed at the bottom, and the plug stuck to the side-wall.

Without efficient removal of interfering lipids, photometric error is almost inevitable. Introducing LipoClear treatment as the first step in the process is straightforward and cost effective. Easily stored and ready for use, keeping a pack in the fridge gives complete confidence in test results – even from those unexpected, yet sadly inevitable, lipaemic and haemolysed samples.