UriStain
Vet Lab Supplies > Laboratory Consumables

UriStain

UriStain™ - for the Microscopic Examination of Urine Sediment

UriStain™ is a urine sediment stain, intended for staining centrifuged urine sediment and used for the examination of WBCs, RBCs, bacteria, yeast and casts. The stain contains various dyes that aid in differentiating the abnormal cellular elements found in urine. UriStain is a ‘one solution’ modification of the Sternheimer and Malvin procedure which compares directly to the Sedi-Stain product.

Sediment Stain – A Valuable Diagnostic Technique

Microscopic examination of urinary sediment is a rapid, easy-to-perform diagnostic procedure that can provide important information to the clinician. Aside from taking an actual tissue biopsy of the kidney, it is widely recognised that the microscopic findings of the urinary sediment are a reliable and valuable diagnostic technique which will give a clear indicator of intrinsic renal disease.

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Long Shelf-life, No Filtration Required

UriStain™ is a stabilised modification of the Sternheimer - Malbin Urine Sediment Stain for the easy differentiation of red and white cells.

  • Red cells - Stains pink
  • White cells - Stains purple
  • Cytoplasm - Stains red/purple
  • Yeast and fungal mycelia – Stains purple
  • Casts - Stains pink to red
  • Epithelial cells - Stains purple
  • Bacteria when alive - Stains red
  • Bacteria when dead - Stains purple
  • Trichomonas - colourless or light blue

For the full Interpretation of results, and the complete procedure, see ‘Instructions for Use’.

Unlike the original formulation of urine staining methods which involved filtration, UriStain requires no filtration and has a long shelf-life. Standardising your collection, evaluation techniques and reporting procedures is of the highest importance.

Reagent Formula

This stain is made from certified dyes which include: Ammonium Oxalate, Safranin, Crystal Violet, Ethanol and Deionized water. Our UriStain™ is available in 15ml dropper bottles and is priced very competitively.

Other items required, include the standard microbiological supplies and equipment such as…

Microscope
Centrifuge
Microscope Consumables 
Sample Collection Tubes 

Stains

Rapi-Diff & Other Ready to Use Stains and Reagents

We supply the very popular Rapi-Diff Stain Set along with the individual stains, A, B and C which can also be purchased separately from us. We also offer a selection of gram stain liquids which include: Acetone for use as a clearing agent, Giemsa, Leishman, Loffler’s Methylene Blue and New Methylene Blue.

We also supply: TB stains, Phosphate Buffered Saline pH 6.8 to support our Rapi-Diff 11 stain, Buffered Formalin, White Cell Counting Fluid and finally, the indispensable Potassium Hydroxide solution for the diagnosis of mites and ringworm in hair and skin samples. If there are any other particular stains that you cannot see in our price tab, please let us know and we will do our very best to source them for you.

Rapi-Diff Stain 11 Stain Set

Our Rapi-Diff II Stain Set which is useful for staining smears, especially blood smears is available in both 500ml and 250ml sets or single solutions. This triple set is for the rapid differential evaluation of haematology and cytological smears, and is directly comparable to DIFF-QUICK and the results are similar to Romanowsky stained smears.

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How to use Rapi-Diff 11

Briefly, the smear is air dried and then immersed in “Solution A” fixing solution (Thiazine dye in methanol) for a least 5 seconds or longer. Then holding the microscope slide at one end, the smear is dipped in and out of “Solution B” (Eosin Y in phosphate buffer) five times allowing 1-2 seconds per dip. After the last dip, the excess stain is drained off onto absorbent paper and rinsed off with phosphate buffer pH 6.8. The process is repeated in “Solution C” (Polychromed Methylene Blue in phosphate buffer) to give the smear a purple colour, washed with buffer and air dried before being examined under the microscope. With this procedure, red/blue staining intensities can be adjusted by varying the number of dips in Solution B or C.

To see our Microscope consumables follow this link

Histology Pots & Buckets

Histology Pots and Buckets, Tough & Leak-proof

Vetlab Supplies’ large histology pots are made of tough polypropylene and are supplied with strong plastic leak-proof lids. These pots have a wide-mouth and base, making them especially ideal for containing and transporting large tissue samples for histopathology.

Clear Polypropylene with Veterinary ID Labels

Our histology pots are particularly useful for allowing large tumours to be completely removed, safely fixed and transported; allowing the pathologist to provide a more accurate diagnosis and prognosis.

Histology Pots - Specifications

Outside measurements are taken from the base to the top of the lid

  • 20ml - Height 4.2 cm - Diameter 3.5 cm
  • 40ml - Height 4 cm - Diameter 5cm
  • 60ml - Height 7.5cm - Diameter 5cm
  • 120ml - Height 8cm - Diameter 5.5cm
  • 250ml - Height 6cm - Diameter 9.5cm
  • 500ml – Height 11cm – Diameter 9cm
  • 1000ml – Height 14cm – Diameter 11cm

ALSO AVAILABLE: 5 Litre White Round Histology Bucket with Push Lid. Height 19.4cm - Diameter 23.6cm

These Specimen/Histology Buckets have a push lid, plastic handle and a tamper evident security tab. The bucket will hold a total weight of 141.0g with a brimful capacity of 5700ml.  These buckets are ideal for collecting larger samples and can be easily stored as they are stackable. Finally, our Neutral Buffered Formalin 10% is available in 1 and 5-litre sizes.

To see our range of Pre-filled Histology Pots 25ml to 120ml follow this link

StatSpin Products

StatSpin Consumables and Accessories

StatSpin 40mm Capillary Tubes

StatSpin 40mm capillary tubes are used for the determination of Haematocrit (Packed Cell Volume – PCV) tests. These capillary tubes are manufactured in high-quality glass and are available either in plain or pre-coated with heparin. The fill volume for these 40mm tubes is 9.4μl.

Capillaries are commonly used to extract small blood samples directly from the vein by using a Surgicutt lancet. Our centrifuge consumable range includes both 75mm and 40mm capillary tubes. This type of tube is not only used for Packed Cell Volume tests, but also the extremely useful function of transporting small volumes of sample to other devices, such as microscope slides for making blood smears and Petri dishes for other tests.

StatSpin Centrifuge Consumables

We also supply StatSpin sealant trays and the StatSpin reader card which is made from tough washable laminated plastic. The reader card has a sliding transparent tube holder to read 40mm capillary tubes by using the sliding percent haematocrit scale provided on the card.

In addition, we also supply the StatSpin OvaTube for processing faecal samples. The StatSpin OvaTube is the first centrifugation method that is easy and fast without any mess. Just add the sample to the tube and mix with standard flotation fluid. Centrifuge in either a fixed angle or horizontal centrifuge, remove the tube, add a coverslip, and twist. In just a few minutes results are ready to read.

StatSpin Rotors

We offer both rotors for StatSpin VT centrifuge. The RT12, 2-Place Microtube Rotor and the RH12, 12-Place Microhaematocrit Rotor. These rotors are lightweight, precision fabricated and pre-balanced for a reliable, high-speed performance.

To see our other Centrifuge Consumables, follow this link

 

StatSpin OvaTube

Ova and Parasite Detection System

In-Clinic Parasite Detection Test Kit

OvaTube is the ideal in-clinic parasite detection test kit which is suitable for companion and small animals and has been developed for use in veterinary hospitals, practices and reference laboratories. In a situation where an animal has internal parasites or a particular parasite, the concentration flotation technique is recommended, particularly the centrifugal technique, for the maximum yield of ova and oocysts.

Worm Egg Detection Method For Companion And Small Animals

The basic principle uses a flotation solution of special Specific Gravity in which the faecal sample is mixed and poured into a centrifuge tube. This can then be incubated vertically on the bench for up to 10-15 minutes. However, it is proven that if the tube is centrifuged at slow speed for a few minutes, the accuracy is greatly improved. Importantly, centrifugation forces heavy debris to the bottom of the tube; allows flotation of the ova and prevents debris from obscuring the final microscopic examination. OvaTube together with our ready-made Vetlab Flotation Solution is ideal for performing both techniques.

OvaTube - The Perfect In-Clinic Solution: fast easy and clean

The StatSpin OvaTube is the first centrifugation method that is easy and fast without any mess, with a simple coring tool to obtain a sample. Just add the sample to the tube and mix with standard flotation fluid. Centrifuge in either a fixed angle or horizontal centrifuge, remove the tube, add a coverslip, and twist. In just a few minutes results are ready to read.

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OvaTube - Advantages

  • Accurate in-clinic results (gold standard)
  • Cost-effective
  • Cleaner processing of samples
  • 3 minute coverslip transfer for microscopic examination
  • Fast results - Diagnose patients whilst they wait
  • Standardises the method

Using StatSpin OvaTube without a Centrifuge

Follow the instructions carefully, then stand the OvaTube vertically in a rack for 10 -15 minutes.

Statspin Ovatube Manual Method

Summary

Centrifugal flotation is recommended by the Companion Animal Parasite Council (CAPC) as the prefered method for routine screening of faecal samples for the detection of parasite ova. In addition, it is recommended that faecal examinations should be performed 2 - 4 times during the first year of life and 1 - 2 times per year in adult pets.

References

Companion Animal Parasite Council (CAPC). 2009.

Comparison Study of StatSpin OvaTube and Standard Centrifugal Method for Recovery of Faecal Parasite and Ova Detection

  Companion Animal Parasitology Council

 

Transfer Pipettes, Pipette Tips

Transfer Pipettes - Disposable Plastic

Plastic disposable transfer pipettes offer a quick, cheap method of transferring or dispensing small volumes of liquids. Our standard transfer pipette has an integral plastic bulb for fast, easy filling and dispensing and a 1ml stem graduated in increments of approximately 0.25ml. In addition, we also supply a larger 3ml transfer pipette and both sizes are supplied in boxes of 500.

Fine Tip Pipettes 

We also supply a similar 1ml, ungraduated transfer pipette with a very fine tip which is ideal for very small volumes of sample or liquids. The fine tip pipettes are supplied in boxes of 400 and all of our products are extremely competitively priced.

Pipette Tips (Finntip & Eppendorf)

These disposable pipette tips are for use with hand-held automatic, air-displacement pipettors. We supply Finntip 1-5ml white tips, Finntip 200-1000µl blue tips and 5-200µl Eppendorf yellow tips. These tips are principally for use with Finn Pipettors, however, they can also be used with other makes of similar pipettor. We are always happy to send samples, ensuring the tips are the correct fit before you make a purchase.

To see our range of Microscope Consumables, follow this link

To see our range of Universal Containers, follow this link

LipoClear Tubes

LipoClear - Lipaemic Clearing Reagent

Background

Lipaemia, or gross elevations of serum/plasma lipoproteins, can be instantly recognised by turbidity in the sample which can cause interference in some analytical methodologies using photometric measurement principles.

Removal of lipaemia can be difficult and complicated by conventional methods. LipoClear tubes, however, offer a quick, simple and safe solution for removing lipaemia without affecting the measurement of other major analytes including enzymes.

Using LipoClear Tubes

LipoClear Tubes are 1.5ml microcentrifuge tubes pre-filled with the LipoClear reagent. LipoClear is a convenient, non-toxic, non-carcinogenic liquid polymer reagent which clears lipaemic serum or plasma after mixing and centrifugation.

Simple Procedure

The simple procedure involves pipetting 0.5ml of sample into a LipoClear Tube, mixing and leaving for 5 minutes at room temperature, and then centrifuging. Ideally, centrifuges spinning at 10,000G or more can be used and will allow separation and clearing in 2 minutes. Low-speed centrifuge, for example, 2000G will need 20 minutes for spinning LipoClear Tubes.

After centrifuging, the cleared sample must be removed for analysis. However, please note that all results must be multiplied by 1.2 to compensate for the sample dilution. This is a refrigerated product and is supplied in pack sizes of 10 or 40 1.5ml microcentrifuge tubes.

To see our range of blood collection tubes follow this link

Faecal Flotation Solutions

Ready-made Faecal Flotation Solutions

Standard S.G. or Made to your Specific Requirements

(Sodium Nitrate & Zinc Sulphate)

Livestock & Companion Animals - Parasitology

Examination of faeces from livestock and companion animals for parasite eggs and oocysts is an important diagnostic procedure in most veterinary practices and laboratories. One of the most common techniques uses the concept of floating parasite eggs to the surface of a special solution in order to be detected under the microscope.

Specific Gravity

Basically, this is achieved by increasing the density or specific gravity (S.G.) by dissolving certain salts in water. The S.G. has to be optimised in order to allow parasite eggs to float and faecal debris to sink. It is generally recognised that the S.G. of faecal flotation solutions should be within the range 1.18 to 1.27 to detect most (but not all) ova.

Vetlab Supplies Ready-made Faecal Flotation Solutions

We offer ready-made faecal flotation solution of either Sodium Nitrate or Zinc Sulphate both of S.G. of 1.200. These solutions are the most popular and recommended for general use. We offer both solutions to accommodate personal choice, although we find that our Sodium Nitrate Solution has less shrinkage effect on Giardia Cysts. However, we can make solutions of different S.G. values to personal order. These solutions are ideal for use with our 'OvaTube' and McMaster slides.

Centrifuge Consumables

Centrifuge Consumables and Accessories

75mm Capillary Tubes - Glass

Microhaematocrit (Mhct) or Packed Cell Volume (PCV) tests require the use of good quality capillary tubes manufactured with a good quality glass. Capillary tubes are either plain or pre-coated with heparin, and are commonly used to extract small blood samples directly from the vein by using a Surgicutt lancet.  Our centrifuge consumables range includes both 40mm and 75mm capillary tubes. This type of tube is not only used for Packed Cell Volume tests, but also the extremely useful function of transporting small volumes of sample to other devices, such as microscope slides for making blood smears and Petri dishes for other tests.

Why Is The Quality Of Capillary Tubes So Important?

It is very important that capillary tubes are manufactured correctly using a good quality material with a consistent bore diameter. Furthermore, the thickness of the wall must not only be strong enough to withstand centrifugal G-forces of up to 10,000 RMP but also thin enough to allow easy viewing of the reading. Finally, a good quality capillary tube should not break when pushed down into the sealant tray, and neither should the sealant plug become detached from the tube. See our Top Tips below.

Centrifuge Consumables Product Range

The length of your capillary tubes will depend on the make of your centrifuge. We supply the Marienfeld Superior Glass brand of 75mm capillary tubes which fit our CombiSpin centrifuge as well as other standard size centrifuges such as Hawksley and Centurion. Our StatSpin 40mm capillary tubes fit our MiniSpin range of benchtop mini centrifuges as well as the StatSpin VT. Our full range of centrifuge consumables and accessories includes quality sealant trays; microcentrifuge tubes, blood collection tubes; readers and our popular Vet-Tube racks (available in a range of bright colours) to hold your samples.

For StatSpin Products follow this link

For Blood Collection Tubes follow this link

To See Our Range Of Centrifuges follow this link

 

Transport Swabs

Transport Swabs - Standard & Mini-tip

(With Amies Transport Medium, Charcoal)

Laboratory transport swabs for sample collection, preservation and transport prior to culture for bacteria and fungi. We supply swabs containing Amies Transport Medium with Charcoal in either a Standard tip or Mini-tip size. This transport medium is useful for preserving fastidious organisms (e.g. C.E.M. organism, Campylobacter spp.) in transit to the laboratory prior to culture.  The charcoal additive is particularly useful in neutralising any toxic metabolic compounds produced by bacterial growth.

Plain Swabs - Standard & Mini-tip

(Without Transport Medium)

We also supply PLAIN swabs without transport medium. Offering both Standard (rayon tip) and Mini-tip (cotton tip) with a plasticised stick and supplied in individual polypropylene tubes. The rayon/cotton tip is non-toxic to most micro-organisms and gives superior performance.

Finally, all of our swabs are available in convenient pack sizes: units of 10, 50 or in larger units of 100.