Description
For Clearing Lipaemic Samples
Note: CataKlear™ replaces LipoClear which has been discontinued:
Introduction
Gross elevations of serum or plasma lipoproteins (lipaemia) cause turbidity in the sample which can interfere with the accuracy of the assay of some analytes which use colorimetric methods. CataKlear™ is a lipid clearing reagent.
The removal of this interfering lipaemia can be achieved by several techniques which include ultracentrifugation (the best method) and solvent extraction. However, these methods have practical disadvantages, including large sample requirements, the use of a high-speed centrifuge or the use of toxic solvents.
CataKlear™ Lipid Clearing agent has been developed to solve this problem without interfering with the accuracy of most tests.
More Info
Method Principle
CataKlear™ is a stable, liquid colloidal nanoparticle dispersion of selectively sized silicone oxides, various surfactants and buffering components. The lipid components in serum/plasma are released through the activity of the surfactants and attach to the silicone oxide nanoparticles. Upon centrifugation, these lipid-silicone oxide nanoparticles are deposited on the bottom of the centrifuge tube leaving the clear, lipid-free serum/plasma layer ready for decanting and testing.
Reagent Presentation
CataKlear™ is supplied in packs of 1 x 12.5ml CataKlear Reagent & 40 microcentrifuge tubes
Shelf-life & Storage
CataKlear can be stored at room temperature 15-230°C (not in direct sunlight). Cold or cool storage (2-15°C) is also acceptable. If stored in the fridge please bring to room temperature before use. DO NOT Freeze or store at below 0°C. When stored as directed, the reagent is stable until the expiration date stated on the label.
Samples: Serum/Plasma
Lipaemic serum is the sample of choice, although lithium heparin plasma samples can also be used. However, the user should be aware of the tests this anti-coagulant can affect.
- Pipette, to each empty micro centrifuge tube, 50 µL of CataKlear concentrate from the bottle.
- Pipette 1ml serum sample (or less if necessary) into tube, mix well and cap the tube. Let stand for 10 minutes at room temperature.
- Centrifuge the tube at speeds of 5,000G or more for 15 mins until the sample has been cleared of lipaemia and the fat has been deposited in a tight button on the bottom of the tube.
- Please Note: It is advisable for each user to establish their own procedure because centrifuges’ maximum speeds can vary.
- Carefully remove supernatant and place in a separate tube ready for testing.
- Finally, all results should be multiplied by the dilution factor caused by the addition of CataKlear to the serum sample.
Limitations and Quality Control
Research has shown that CataKlear™ is unlikely to interfere with the assay of the following analytes: Albumin, ALP, ALT, Amylase, Creatinine, Phosphate and Urea. Details of a study are available on request. However, because users will be using a wide variety of reagents and analysers, we strongly recommend that each laboratory establish performance characteristics of CataKlear™ with their own analytical system. Please see below:
Precautions and Safety
Avoid contact of reagent with skin and eyes. If contact occurs, wash affected area with copious amounts of cold water. Above all, if you have any queries regarding the use of CataKlear™ Lipid Clearing Agent please telephone our Technical Services Dept. on 01798 874567.
To see our full range of sample collection tubes…follow this link
CataKlear Health and Safety Data Sheet
CataKlear Health and Safety Data Sheet
SDS CataKlear REV-3.0 (1) 2023
