LipoClear – Lipaemic Clearing Reagent
Lipaemia, or gross elevations of serum/plasma lipoproteins, can be instantly recognised by turbidity in the sample which can cause interference in some analytical methodologies using photometric measurement principles.
Removal of lipaemia can be difficult and complicated by conventional methods. LipoClear tubes, however, offer a quick, simple and safe solution for removing lipaemia without affecting the measurement of other major analytes including enzymes.
Using LipoClear Tubes
LipoClear Tubes are 1.5ml microcentrifuge tubes pre-filled with the LipoClear reagent. LipoClear is a convenient, non-toxic, non-carcinogenic liquid polymer reagent which clears lipaemic serum or plasma after mixing and centrifugation.
The simple procedure involves pipetting 0.5ml of sample into a LipoClear Tube, mixing and leaving for 5 minutes at room temperature, and then centrifuging. Ideally, centrifuges spinning at 10,000G or more can be used and will allow separation and clearing in 2 minutes. Low-speed centrifuge, for example, 2000G will need 20 minutes for spinning LipoClear Tubes.
After centrifuging, the cleared sample must be removed for analysis. However, please note that all results must be multiplied by 1.2 to compensate for the sample dilution. This is a refrigerated product and is supplied in pack sizes of 10 or 40 1.5ml microcentrifuge tubes.
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